RNA-seq of hemoglobin depleted polar bear whole blood transcriptome - SRA

SRX5243176: RNA-seq of hemoglobin depleted polar bear whole blood transcriptome
1 ILLUMINA (HiSeq X Ten) run: 58.1M spots, 17.5G bases, 6.6Gb downloads

Design: Modified Smart-seq2 protocol. Full-length cDNA generated using oligodT priming and template switching, depleted of hemoglobin transcripts using Long-DASH protocol, and fragmented for Illumina sequencing using Tn5 enzyme and Nextera oligos.

Submitted by: (UCSC)

Study: Hemoglobin depleted polar bear whole blood transcriptome

PRJNA514749 • SRP178553 • All experiments • All runs

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Smart-seq2 Illumina and R2C2 ONT data for polar bear transcripts. Input full-length cDNA was depleted of hemoglobin using Long-DASH method.

Sample:

SAMN10734297 • SRS4245857 • All experiments • All runs

Organism: Ursus maritimus

Library:

Name: PB19_undepleted_Smart-seq2

Instrument: HiSeq X Ten

Strategy: RNA-Seq